- Vortex + benchtop centrifuge
- Horizontal vortex with holder for Sterivex (15 mL tube holders)
- Centrifuge for 2 mL tubes (relative centrifugal force needed: 11,000 x g)
- Incubator (temperature needed: 70°C)
- MagnetaPure 32 Nucleic Acid Purification System (Dutscher)
- Specific DNA-workstation (sterile area equipped with air filtration and UV systems)
All tubes and tips must be sterile
- 1000 µL tips with filter
- 100 µL tips with filter
- 50 mL tubes: to prepare aliquots
- 5 mL tubes: 1 per 8 samples to prepare NucleoMag B-Beads and MWA2 mix
- 2 mL tubes: 1 per sample to transfer lysate + 2 to prepare aliquots
- 1.5 mL tubes: 1 per sample to transfer eluted DNA
- 5 mL syringes with Luer lock tip
- 96-well plate with 2 mL deep-wells, U-Bottom (Macherey Nagel - 746032.DEEP): 1 per 16 samples
- Magnetic rod coverfor MagnetaPure 32 (Macherey Nagel – 747032.TC): 1 per 8 samples
- Plastic film to protect the 96-well plate
For any manipulation in a rare DNA room, provide complete equipment (disposable coat, cap, mask, shoe covers & gloves).
- NucleoMag DNA/RNA Water kit (Macherey Nagel)
Note: shelf life of reagents for 24 months from production
- Buffer C1 (Macherey Nagel)
Note: Buffer C1 for lysis of Sterivex filtered samples is not supplied in the kit and must be ordered separately from the supplier (900 µL per sample)
- Sterivex filter units prefilled with preservation buffer (e.g. C1) or not