Oct 05, 2022
E. coli growth trend measurement (culture with silver ion)
- 1XJTLU
Protocol Citation: Weizhuo.Chen, An.Huang 2022. E. coli growth trend measurement (culture with silver ion). protocols.io https://dx.doi.org/10.17504/protocols.io.81wgby1byvpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 17, 2022
Last Modified: October 05, 2022
Protocol Integer ID: 70181
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Abstract
This protocol helps obtain growth curve of E. coli cultured with concentration gradient of silver ion.
IPTG induction
IPTG induction
Grow Escherichia coli overnight and dilute the culture using LB to OD600=0.4-0.6.
Add IPTG to cell culture (final concentration 1mM), and incubate the culture 3h at 171 rpm, 37℃ in orbital shaking incubator.
Sample addition to microplate wells
Sample addition to microplate wells
Cells cultures, LB broth and silver nitrate (150μM) are added into 96-well microplate. The recipe is as the following chart:
E. coli cultured with concentration gradient of silver ion.
Microplate reader measurement
Microplate reader measurement
Incubate the microplate in orbital incubator (37℃ 171 rpm) and measure OD600 by microplate reader every two hours for two days.