Oct 17, 2022
Drug feeding protocol
- 1Brigham and Women's Hospital and Harvard Medical School
Protocol Citation: Mel Feany 2022. Drug feeding protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8j9n9g2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 17, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 71452
Keywords: ASAPCRN
Abstract
This protocol covers the steps for drug feeding in fly models.
Collect flies for feeding:
-Clear bottles the day before collecting
-Select appropriate genotype for feeding
Prepare drugs to be fed:
-Thaw drugs
-Add 3 mL water to each vial
-Add appropriate number of ul drug to water, mix by swirling
Make fly food in vials that contain water and drug:
-Place the label that matches the drug you are using on the vial
-Add dry fly food to make a thick slurry, water will be absorbed in minutes
-Turn on gas and knock out flies. Put up to 40 flies into vial.
-Put the flies in a box in the 25 °C incubator
Flies are fed 3 doses of each drug. Flies are fed for a total of 10 days.
Harvest flies at 10 days and fix in formalin for histological analysis or process appropriately for other studies as needed.