Typically, 0.5uL of 1pM barcode will give approximately 1 barcode for every 10 droplets. However, it is best to measure the barcode encapsulation rate by making PCR droplets containing the barcodes at the expected dilution and P7 and Barrev primers targeting the barcode for amplification. Visualize the resulting PCR emulsion using SYBRgreen staining under the microscope to obtain the real encapsulation ratio. Typically, the real barcode concentration can be ~5 fold off from the expected concentration based on manufacturer's labelling.