Apr 28, 2022
Donor eligibility Criteria for Human Liver for HuBMAP TTD-Columbia/PSU
- Presha Rajbhandari1,
- Hua Tian2,
- Helen Remotti1,
- Brent R Stockwell1
- 1Columbia University, New York, NY;
- 2Pennsylvania State University
Protocol Citation: Presha Rajbhandari, Hua Tian, Helen Remotti, Brent R Stockwell 2022. Donor eligibility Criteria for Human Liver for HuBMAP TTD-Columbia/PSU. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzy5wrlx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 28, 2022
Last Modified: April 28, 2022
Protocol Integer ID: 61626
Abstract
This documents outline the criteria for human liver tissue donor eligibility for the Hubmap BioMolecular Atlas Program (HuBMAP), Transformative Technology Development (TTD) Center at Columbia University/Pennsylvania State University. Samples include those from liver resections/biopsies/transplantation. Donor eligibility, consent for tissue donation, documentation on donor medical history and sample storage is performed by the Columbia University Biobank.
Inclusion criteria for Donor:
Inclusion criteria for Donor:
-Age range >18
-BMI <29
-All race, ethnicity and gender (equal male and female) are accepted
-Negative for HIV, Hepatitis B or C virus, CMV or EBV, COVID-19, sepsis
-Negative for liver diseases including fatty liver disease, hepatocellular carcinoma, and diabetes
Sample:
Sample:
-Visual inspection of liver for any abnormalities
-Site of tissue far from benign lesions for resection cases
-If available, recover at least 1cm x 1cm x 1cm of liver and flash freeze as quickly as possible on dry ice /isopentane slurry (without OCT) and store at -80C
-Warm ischemia time <30 minutes and/or cold ischemia time < 2 hours
-Normal histology and tissue quality assessed by hematoxylin and eosin staining by expert pathologist
Sample information:
Sample information:
The metadata related to the sample to include:
-Pathogenesis/diagnosis for resected tissue or donor cause of death
-Donor age
-Demographics
-Medications and dose
-Serological test results including HepB, HepC, HIV, CMV, EBV
-Past medical history
-Smoking/Drug use/Alcohol use
-Time from brain death, if deceased liver donors