Mar 08, 2024

Public workspaceDOH Workshop Protocol Parts 1, 2 and 3 V.1

DOI
dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
  • 1The Roslin Institute, University of Edinburgh
Bryan Wee
Open access
DOI: dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
Collection Citation: Vesa Qarkaxhija, Bryan Wee, Natalie Ring 2024. DOH Workshop Protocol Parts 1, 2 and 3. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: March 06, 2024
Last Modified: March 08, 2024
Collection Integer ID: 96355
Funders Acknowledgement:
Royal Society
Grant ID: RGS\R1\231137
Abstract
This collection of protocols details the extraction of high-molecular-weight DNA from Gram-negative bacterial culture, purification of dsDNA, removing contaminants and low molecular weight DNA, and creation of Nanopore sequencing libraries for the MinION flow cell.
Files
Protocol
DOH Workshop Protocol Part 1: Purification of High- Molecular Weight Genomic DNA from Gram-Negative Bacteria (MagAttract HMW DNA)
Name
DOH Workshop Protocol Part 1: Purification of High- Molecular Weight Genomic DNA from Gram-Negative Bacteria (MagAttract HMW DNA)
Version 1
Bryan Wee
Bryan WeeThe University of Edinburgh
Protocol
DOH Workshop Part 2: Promega Pronex protocol
Name
DOH Workshop Part 2: Promega Pronex protocol
Version 1
Bryan Wee
Bryan WeeThe University of Edinburgh
Protocol
DOH Workshop Protocol Part 3: Library preparation for Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24) with Pronex modification
Name
DOH Workshop Protocol Part 3: Library preparation for Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24) with Pronex modification
Version 1
Bryan Wee
Bryan WeeThe University of Edinburgh