Plate Well | volume of TE (uL) | volume (uL) of 2ug/mL stock DNA | volume of diluted picog dye (uL) | final DNA concentration in assay (ng/mL) | total DNA in well (ng) | |
A1 & A2 | 0.00 | 100.00 | 100 | 1000 | 200 | |
B1 & B2 | 90.00 | 10.00 | 100 | 100 | 20 | |
C1 & C2 | 99.00 | 1.00 | 100 | 10 | 2 | |
D1 & D2 | 100.00 | 0 | 100 | 0 | 0 |
Plate Well | volume of TE (uL) | volume (uL) of 0.5ug/mL stock DNA | volume of diluted pg reagent (uL) | final DNA concentration in assay (ng/mL) | total DNA in well (ng) | |
A1 & A2 | 0.00 | 100.00 | 100 | 250 | 50 | |
B1 & B2 | 90.00 | 10.00 | 100 | 25 | 5 | |
C1 & C2 | 99.00 | 1.00 | 100 | 2.5 | 0.5 | |
D1 & D2 | 100.00 | 0 | 100 | 0 | 0 |
Plate Well | volume of TE (uL) | volume (uL) of 0.05ug/mL stock DNA | volume of diluted pg reagent (uL) | final DNA concentration in assay (ng/mL) | total DNA in well (ng) | |
A1 & A2 | 0.00 | 100.00 | 100 | 25 | 5 | |
B1 & B2 | 90.00 | 10.00 | 100 | 2.5 | 0.5 | |
C1 & C2 | 99.00 | 1.00 | 100 | 0.25 | 0.05 | |
D1 & D2 | 100.00 | 0 | 100 | 0 | 0 |