Prepare sampling kit (sylphium filter, gloves, ATL buffer, syringe)
Find an interesting of environmental site.
Inject 1-3 L of environmental water into sylphium filter using a clean sterile beaker and 50 ml syringe.
Inject a volume of 3 ml ATL buffer (Qiagen) in the sylphium filter to be stored at room temperature until it is transferred and processed in the laboratory.
Sylphium filters were stored at 4 °C once they arrived to laboratory.
Incubate Sylphium filter at 56°C for 30 min to dissolve any crystalls of ATL buffer.
Transfer the buffer from the sylphium filter capsule using 3 ml syringe into a 15 mL falcon tube.
Be careful not to apply too much pressure when collecting lysate from sylphium filter.
The volume of lysate might vary between 2-3 ml.
Add proteinase K with the ratio 10 µl for 100 µl lysate, Vortex for a few seconds and incubate at 56°C overnight.