Mar 02, 2022

Public workspaceDNA Extraction from Water Samples V.1

This protocol is a draft, published without a DOI.
  • 1New Mexico State University
Andrew Dominguez
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Protocol Citation: Andrew Dominguez, Yanyan Zhang, Nicole Pietrasiak 2022. DNA Extraction from Water Samples. protocols.io https://protocols.io/view/dna-extraction-from-water-samples-b4smqwc6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: February 07, 2022
Last Modified: March 02, 2022
Protocol Integer ID: 57901
Abstract
This protocol was followed to extract DNA from potable and tailored water samples used to water various turf grasses in a greenhouse study. We closely followed the extraction protocol provided in the Qiagen DNeasy PowerWater Kit with a few modifications to account for low product yield.
Protocol materials
ReagentDNeasy PowerWater KitQiagenCatalog #14900-50-NF
Step 2
ReagentWhatman ME 24/21 STL Membrane Filters white (Mixed cellulose ester) black gridMerck MilliporeSigma (Sigma-Aldrich)Catalog #10408712
Step 2
Sample Prep
Sample Prep
If water samples are stored at Temperature-80 °C allow time for samples to defrost depending on the volume. Large volumes can take a few hours to defrost.

DNA Extraction
DNA Extraction
2h
2h
DNA extraction was completed using ReagentDNeasy PowerWater KitSigma AldrichCatalog #14900-50-NF with the following modifications:
  • In step 1, the specific filters used were ReagentWhatman ME 24/21 STL Membrane Filters white (Mixed cellulose ester) black gridSigma AldrichCatalog #10408712
  • In step 21, Amount30 µL of Solution EB was added to increase DNA concentration
  • Following step 21 the wait time was Duration00:05:00 before moving on to step 22
  • Following step 23, DNA extracts were stored at Temperature-80 °C




2h
A demonstration for folding a filter and inserting it into the Bead Tube can be found here.