This protocol requires the use of a Biological Safety Laboratory level S2 due to handling of human substrates.
This protocol takes ~1 day.
Prepare a cool tube-rack for 1.5ml and 2ml tubes by placing it at +4°C for the DNA-clean up. This will speed up reaction time with a 'cold start'.
Modern dental plaque samples should be stored at least at +4°C or preferably at -20°C to preserved DNA after sampling.
Check waste disposal guidance for all reagents in this protocol against your corresponding laboratory regulations.
Make sure all necessary equipment is available (see Materials).
HPLC = High Performance Liquid Chromatography (i.e., HPLC-grade water)
Consider taking along a positive control (sample of known performance) and a negative control (tube with HLPC-grade water instead of DNA) in order to assess the performance of the protocol and the level of background contamination. Take into account these two extra samples in your calculations for buffer preparations.