1. In the RSC Software, press Start to access the extraction method selection screen.
2. Select Cultured Cells DNA method, then press Proceed.
3. Select the lanes being used by touching them. Sample names can be inputted at this time.
Note: For the RSC48, press the "BACK" and "FRONT" buttons on the bottom of the screen to move to each respective rack.
4. Press Proceed to continue. The instrument door will open.
5. Install the deck tray(s) into the platform by angling the back of the tray and inserting first. Press down the front of the tray until it snaps into place. Ensure the deck tray is level and fully seated.
Note: For the RSC48, ensure the tray labelled FRONT and BACK are placed in the correct places in the instrument.
6. Verify that all of the cartridges are properly inserted into the tray, the elution tubes are present and uncapped, and then plungers are in Well 8.
Note: Ensure there are no old plungers left on the instrument from a prior run.
7. Press Start to begin the extraction run.
8. Follow on-screen instructions at the end of the run.
9. Remove the deck tray(s) from the instrument. The DNA can be transferred to 1.5 ml LoBind Tubes for storage.
10. Remove the cartridges and plungers from the deck trays and discard in a biohazard container.
11. Clean the tray with 70% Ethanol. Do NOT use bleach to clean the trays as this reacts with Guanidine Thiocyanate.
12. Proceed to the Cleaning and Decontamination steps.
13. Proceed to quantify the DNA using the DNA Quantification using the Qubit Fluorometer SOP.