Jan 05, 2023

Public workspaceDNA Extraction from Arabidopsis thaliana V.1

DOI
dx.doi.org/10.17504/protocols.io.4r3l276zqg1y/v1
  • lilyli1
  • 1CAU
Lily Li
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DOI: dx.doi.org/10.17504/protocols.io.4r3l276zqg1y/v1
Protocol Citation: lilyli 2023. DNA Extraction from Arabidopsis thaliana. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l276zqg1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 04, 2023
Last Modified: January 05, 2023
Protocol Integer ID: 74719
Abstract
This is a protocol for extract DNA from arabidopsis thialiana.
Add 400 mL DNA extraction solution into Amount1.5 mL centrifuge tube.

Grind 3 small leaves or 1 large leaf with a grinding stick in the centrifuge tube with the extract.
Shake the oscillator for Duration00:00:05 , and place it at room temperature until other preparations are completed.

5s
Centrifuge at 16 000 rpm for 2 minutes at room temperature.
Transfer 200 mL of centrifuged supernatant into a clean centrifuge tube.
Add 200 mL isopropanol, mix well and place at room temperature for 3 minutes.
Centrifuge at 16 000 rpm at room temperature for 5 minutes, remove the supernatant, wash the precipitate with 70% ethanol, and dry the precipitate at room temperature.
Dissolve DNA in 100 mL deionized water and store it in a refrigerator at – 20 ° C.