Place the tissue in a 1.5 ml or 2 ml microcentrifuge tube. Add 300 µl Deparaffinization Solution, vortex vigorously for 10 s, and centrifuge briefly to bring the sample to the bottom of the tube.
Note: For specimens stored only in alcohol (=not fixed in paraffin), "deparaffinization step" is not required. In this case, instead of 300 µl Deparaffinization Solution, add 300 µl ATL, 300 µl AL, and 300 μl ethanol (96–100%), and proceed to the next step 3, without step 2 (incubation).