A | B | C | D | E | F | G | |
Component | Contents | Required | Chemical molarity | per L | per 100 mL | pH | |
Lysis solution 1 | guanidine thiocyanate | 147 mM | 118.16 | 17.33 g | 1.73 g | 9.0 | |
trisodium phosphate | 228 mM | 380.13 | 86.67 g | 8.67 g | - | ||
sodium chloride | 26 mM | 58.44 | 1.5 g | 0.15 g | - | ||
1 M Tris HCl | 67 mM | - | 67 mL | 6.7 mL | - | ||
0.5 M EDTA | 27 mM | - | 53 mL | 5.3 mL | - | ||
Lysis solution 2 | aluminium ammonium sulphate* | 90 mM | 453.33 | 40.8 g | 4.08 g | - | |
SDS | 1.25 % | - | 12.5 g | 1.25 g | - | ||
Protein flocculant | ammonium acetate | 5 M | 77.0825 | 385.41 g | 38.54 g | - | |
Inhibitor flocculant 1 | aluminium ammonium sulphate* | 180 mM | 453.33 | 81.6 g | 8.16 g | - | |
Inhibitor flocculant 2 | calcium chloride dihydrate | 204 mM | 147.01 | 30 g | 3 g | - | |
Binding solution | guanidine HCl | 5.5 M | 95.53 | 525.42 g | 52.54 g | - | |
Wash solution | EtOH | 80 % | - | - | - | - | |
Elution buffer | Pure H2O or 1mM Tris | - | - | - | - | 8.0 |