Reagent | Volume µl (1 rxn) | |
Molecular grade water | 44 | |
1U/µl KAPA Express Extract Enzyme | 1 | |
10X KAPA Express Extract Buffer | 5 |
Temperature (°C) | Time (minutes) | |
30 | 30 | |
60 | 30 | |
95 | 5 | |
4 | ∞ |
Component | Volume (1 x 20µl rxn) | |
KAPA Plant PCR buffer (contains dNTPs) | 10.1 | |
PCR grade water | 2.9 | |
Polymerase | 0.2 | |
MgCl2 (25mM) | 0.4 |