Healthy male Sprague-Dawley rats (RRID: RGD_737903; Envigo RMS, LLC, Indianapolis, IN) (n=6; 2-3 months; weighing: 200-350 g)
Isoflurane (5%; Cat #: 029405, Covetrus North America)
Heparin (0.2 mL; 1000 units/mL, Cat #: 25021-400-10, Sagent Pharmaceuticals)
Paraformaldehyde (Cat #: 416780010, Acros Organics)
Bovine serum albumin (Cat #: A7906, Sigma-Aldrich)
Normal donkey serum (Cat #: 017-000-121, Jackson Immunoresearch)
Triton X-100 (Cat #: T8787, Sigma-Aldrich)
NaN3 (Cat #: S2002, Sigma Aldrich)
Anti-CGRP primary antibody (Rabbit; Cell Signaling, Danvers, MA; Cat # 14959, RRID: AB_2798662; 1:200)
Alexa Fluor 488 secondary antibody (Donkey; Thermo Fisher, Waltham, MA, Cat# A21202, RRID:AB_141607; 1:100)
Methanol (Cat #34860: , Sigma-Aldrich)
DCM (dichloromethane) (Cat #: 270997, Sigma-Aldrich)
Ethanol (Cat #: E7023, Sigma-Aldrich)
Depex mounting medium (Electron Microscopy Sciences #13514)
Zeiss Axio Imager M2 (Item no.: 430004-0000-711)
Leica Confocal Microscope (Type: TCS SP5)