Feb 10, 2023
Version 2
Disposal of Environmental Samples from Water Bodies Infested with Zebra Mussels V.2
- 1Baylor University
Protocol Citation: Malcolm A Barnard, Stephen Powers 2023. Disposal of Environmental Samples from Water Bodies Infested with Zebra Mussels . protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lynoymvx9/v2Version created by Malcolm A Barnard
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 20, 2022
Last Modified: February 10, 2023
Protocol Integer ID: 68955
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Abstract
Zebra Mussels (Dreissena polymorpha) are a highly invasive species that have been invading water bodies around the United States and beyond. This protocol for sample disposal for sample collected from know zebra mussel infested water bodies to prevent spread though local water treatment facilities by bleaching the samples to kill any larvae or other potential zebra mussels in the sample water.
Materials
Bleach (10% Sodium Hypochlorite)
Dishwashing bin to add sample water and bleach
Safety warnings
Do not mix acids and bleach. This will create chlorine gas.
Wear gloves, eye protection, and a lab coat while performing this protocol.
Sample Killing
Sample Killing
4h 5m
4h 5m
Pour all environmental sample water from bottles into a dish pan.
Save the bottles for acid washing
Safety information
Do not add bleach to bottles being cleaned using an acid bath and acid as the combination of acid and bleach produces chlorine gas.
.
Add 50 mL of bleach per 1 L of sample water.
Let the bleached sample sit for a minimum of four hours if not until the next day.
4h
Sample Disposal
Sample Disposal
5m
5m
Dispose of bleached sample down drain with excess water or other waste pathway as described by local environmental health and safety guidelines.
Save bin for future sample disposal or bleach work.