Aug 29, 2024

Public workspaceDFP-induced mitophagy experiments

DOI
dx.doi.org/10.17504/protocols.io.e6nvw11m9lmk/v1
  • 1Sascha Martens lab, University of Vienna, Max Perutz Labs - Vienna
Elias Adriaenssens
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.e6nvw11m9lmk/v1
Protocol Citation: Elias Adriaenssens 2024. DFP-induced mitophagy experiments. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvw11m9lmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 23, 2024
Last Modified: August 29, 2024
Protocol Integer ID: 101114
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-000350
Marie Skłodowska-Curie MSCA Postdoctoral fellowship
Grant ID: 101062916
Abstract
This protocol details the DFP-induced mitophagy experiments.
Materials
Deferiprone (DFP) (379409, Sigma Aldrich)

Reagent3-Hydroxy-1,2-dimethyl-4(1H)-pyridoneMerck MilliporeSigma (Sigma-Aldrich)Catalog #379409

DFP-induced mitophagy
DFP-induced mitophagy
2d
2d
To induce mitophagy,

  • Treat the cells for Duration24:00:00 with Concentration1 millimolar (mM) Deferiprone (DFP) (379409, Sigma Aldrich), an iron chelator that mimics hypoxic conditions through stabilization of the transcription factor HIF1α and subsequent upregulation of NIX and BNIP3.
1d
Freshly dissolve the compound in sterile dH2O on the day itself and boil for Duration00:01:00 at Temperature95 °C to dissolve the compound.
1m
Temperature