LB Broth (HuanKai Microbial, cat. no. 028324)
LB Agar (HuanKai Microbial, cat. no. 028330)
50 × TAE buffer (Solarbio, cat. no. T1060)
Glycerol (Solarbio, cat. no. G8192)
Spectinomycin (Aladdin, cat. no. 64058-48-6)
RNase-free ddH2O (Sangon Biotech, cat. no. B541018-0010)
TIANprep Mini Plasmid Kit (TIANGEN, cat. no. DP103-02)
2 × Phanta Flash Master Mix(Dye Plus) (Vazyme,cat. no. P520-01)
2 × SanTaq PCR Master Mix (with Blue Dye) (Sangon Biotech, cat. no. B532061-0040)
BsaI-HFv2 (NEB, cat. no. R3733).
T4 DNA Ligase (NEB, cat. no. M0202V)
T4 Polynucleotide Kinase (NEB, cat. no. M0201V)
DNA Marker (100-5000 bp) (Sangon Biotech, cat. no. B500351-0500)
DNA Marker (100-2000 bp) (Sangon Biotech, cat. no. B500350-0500)
Agarose (Sangon Biotech, cat. no. A620014)
4S Green Plus Nucleic Acid Stain (BBI, cat. no. A616696-0100)
Eppendorf Micropipettes (0.1-2.5, 2-20, 20-200 and 100-1,000 µL)
Micropipette tips (10, 200 and 1,000 µL)
0.2 mL of 96-well PCR rectangular ice box (Biosharp, cat. no. BC026)
Erlenmeyer flasks (150, 250 and 500 mL)
Laboratory glass bottles (100, 250, 500 and 1,000 mL)
Beakers (500 and 1,000 mL)
Inoculating loop (sterile, 1 µL and 10 µL; Biosharp, cat. no. BS-QT-048 and BS-QT-049)
Inoculation spreader (sterile, L-shaped; Biosharp, cat. no. BS-PS-A)
Petri dishes (ØxH: 90 × 15 mm and 60 × 15 mm, Biosharp, cat. no. BS-90-D and BS-60-D)
Safe-Lock microcentrifuge tubes (1.5 and 2.0 mL; Biosharp, cat. no.BS-15-M-S and BS-20-M)
Centrifuge tubes (10mL, 15 mL and 50 mL; Biosharp, cat. no. BS-100-M, BS-150-M and BS-500-M)
PCR tubes (strips of eight, 0.2 mL; Biosharp, cat. no. BS-0208-T)
MicroPulser electroporation cuvette (0.1 cm gap; Bio-Rad, cat. no. 1652083)
Parafilm (ØxH:4 cm×200 m, junor packaging, cat. no. JUNLE061)
Thermal cycler (C1000 Touch Thermal Cycler, BIO-RAD, cat. no. CT062680)
Microwave (Galanz, cat. no. P70D20N1P-G5(W0))
Electronic Scale (JinXuan, model no. JX.C10002)
Vortex mixer (Scientific Industries, model no. G560E)
Centrifuge (Eppendorf, model no. 5418R)
Incubation shaker (ZHICHENG, model: ZWYC-2932)
MicroPulser Electroporator (Bio-Rad, cat. no. 1652100)
TGreen Plus glue cutter (TIANGEN cat. no. OSE-470L)
OSE-GC (TIANGEN cat. no. RH191448)
MilliQ water purification system (MILLIPORE, USE, cat. no. z00Q0V0T0)
Autoclave tape (Biosharp, cat. No. BS-QT-028)