Oct 25, 2019
Determining Lipid Content in Embryos using Nile Red Fluorescence
- Luciano Bonilla1,
- Peter J Hansen1
- 1University of Florida
External link: https://animal.ifas.ufl.edu/hansen/lab_protocol_docs/detection_lipids_embryos_nile_red_fluorescence.pdf
Protocol Citation: Luciano Bonilla, Peter J Hansen 2019. Determining Lipid Content in Embryos using Nile Red Fluorescence. protocols.io https://dx.doi.org/10.17504/protocols.io.8qwhvxe
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 25, 2019
Last Modified: October 25, 2019
Protocol Integer ID: 29174
Keywords: preimplantation embryo, Nile Red, lipid
Abstract
The following protocol is derived from the protocol described by Genicot et al. (Theriogenology 63: 1181- 1194, 2005) and is based on the fluorescence emitted by Nile Red when in association with lipid (see Fowler and Greenspan, J Histochem Cytochem 33: 833-836, 1985).
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