Several methods can be used, including hexokinase, glucose oxidase or glucose dehydrogenase.
The method used in this work is that of glucose oxidase / peroxidase whose principle is as follows:
Glucose, under the action of glucose-oxidase, is oxidized to gluconic acid with formation of hydrogen peroxide.
In the presence of peroxidase, the hydrogen peroxide produced transforms a colorless reduced chromogen into an oxidized chromogen colored pink whose intensity of coloration measurable by spectrophotometry at 505 nm is proportional to the glucose concentration of the sample.
Glucose + O2 + H2O --------------------------→ Acide gluconique + H2O2
H2O2 + phénol + amino 4 phenazone ----------------------→ Quinone imine + 2 H2O