Jun 24, 2022
Determination of MIC according to the EUCAST E.DEF 11.0 method
- 1AIDS Reference Laboratory, Department of Clinical Microbiology, University Hospital of Liege, 4000 Liege, Belgium
Protocol Citation: Khalid El Moussaoui 2022. Determination of MIC according to the EUCAST E.DEF 11.0 method. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygxz83kv8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Other
We attempted this protocol, but could not get it to work in our workspace
Created: June 24, 2022
Last Modified: June 24, 2022
Protocol Integer ID: 65232
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Abstract
Determination of MIC according to the EUCAST E.DEF 11.0 method.
With the disinfected tweezers, remove the filter (attention: do not take the filters of protections having a slightly bluer tint) and place them in the support provided for this purpose. Close the holder.
Pour the contents of TUBE A (sterile water + Tween) onto the Petri dish containing the starting sample and wipe with a swab until the suspension is relatively cloudy.
Aspirate the suspension with the syringe.
Filter this suspension by injecting it into the filter (0,45 µm) holder and collect the filtered suspension in
suspension in TUBE A.
Measure the density of TUBE A and adjust it to 0.5 McFarland. Then take 500 μL of this
suspension and place it in TUBE B (to be done 2x since we are working in duplicates). TUBE B contains sterile demineralized water (Thermo Fisher T3339).
Place an inoculation head on the tube and inoculate with the Sensititre Aim.
Incubate at room temperature for 7 days and read the result at 492 nm.