Genekam has developed an unique ready to use PCR kit for the detection of SARS CoV-2 indifferent samples. This was developed on 20.01.2020 just at the beginning of the pandemic outbreak. This test is mutation resistant test, hence mutations taking place in different part of this virus is not effecting the performance against many tests available in literature and market, which cannot provide reliable results because the mutations affect them strongly. This test can be used to find the performance of other tests being used in different laboratories in the world because old samples can be tested with this kit only as mutations have no effect. This is reliable and stable along with good sensitivity. Many approved kits from different manufactures were withdrawn from the market as well as some of protocols provided through world organizations have to be modified because of the mutations, but not Genekam FR754 ready to use real time PCR.
Protocols provided from different organisations used 3 different genes of the virus, therefore many users are using a multiplex assay. It is well known that multiplex assay lack the sensitivity as achieved with singleplex test. Our test is a singleplex assay, that does not have the quality problems as faced through such multiplex assay. This targets only one conversative part.
This test is highly specific also along with using on RNA of broader range of samples like buccal and nasal swab, urine, cell culture, lymph nodes, plasma, respiratory tract samples, other body fluids and tissue samples. This is tested with related and non-related targets like RSV, Influenza A strains (H1N1, H3N2, H5N1), Influenza B, CMV, EBV, Salmonella, Chlamydia, Maesles, Staphylococcus aureus, Yellow fever, Zikavirus, Mumps, Rubella, Herpes Simplex Virus 1&2, various healthy human samples ec. These are a few examples.
The test is in position to detect the Wuhan strain and its mutated versions like South African mutations (B.1351 and B 1.1.529), UK mutation (B1.1.7), Brazil mutation (P1), Indian mutation (B1.617), Omicron mutations (B.A.2.86, XBB 1.5 like, XAK), B.1.427, C37 and B.1621 as examples. This shows that this test is mutation resistant!
Sensitivity: 0.000003pg/µl, (0.003fg/µl)