Avian Influenza virus H5N1 has caused a number of outbreaks in human beings in recent years. Recently it has caused outbreak in human and cows in USA. Therefore it is essential to detect the presence of this virus as early as possible in order to monitor the presence of this virus, hence we have developed a number of ready to use PCR kits to detect this virus. Highly pathogenic strains are reponsible for such outbreak. It spreads also in poultry with very fatal consequences. The kit double- and triple-checks the presence of this virus, therefore it makes possible to detect the H5N1 strains circulating in different countries worldwide, moreover mutated versions are likely to be detected with double and triple check method. The cross reaction panel has done with related and non related pathogens like Influenza virus strains: H3N2, H1N1, H7N3, HIN1 (California strain); RSV, Enterovirus, CMV, Influenza B, Mycoplasma etc.