Dec 01, 2023
Version 2
Deepwell Reusual Protocol V.2
- 1UoL
Protocol Citation: Morris Jack 2023. Deepwell Reusual Protocol . protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlko2x6v5r/v2Version created by Morris Jack
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 01, 2023
Last Modified: December 01, 2023
Protocol Integer ID: 91694
Abstract
This is a protocol to reuse deepwells for sustainability practices
Growing cultures in a deep well
Growing cultures in a deep well
Cover a deep well with two layers of aluminium foil and tape the front and back to hold it securely. The tape also turns the aluminium foil into a lid for the deep well by temporarily peeling off the front tape.
Autoclave at 120C for 20min. After autoclaving, keep in a heated oven (> 50C) overnight for drying and desiccation.
Do not fill the deep wells more than 60% of their max volume if they are to be placed in a shaking incubator to avoid spillage.
Deep well Decontamination
Deep well Decontamination
Tape around the deep well over the aluminium foil to prevent spillage.
While carrying the deep well outside the lab space, enclose it in a waterproof autoclave bag. Keep sufficient absorbent paper on hand in case of accidental spillage.
In the autoclave facility, autoclave the deep wells at 120C for >30min to decontaminate.
Once autoclaved, the deep wells will be brought back to the lab
Carefully peel the aluminium foil and dispose the cultures and aluminium foil into a disposal bag containing paper roll to absorb the liquid.
Send decontaminated culture for incineration (yellow bin route).
Rinse the deep well plate with water and a brush to clean before future use.
Keep in a heated oven (> 50C) overnight for drying and desiccation