Jan 13, 2025

Public workspaceDeckLayout: automated in-solution hybridization capture (MPI-EVA method) V.1

DOI
dx.doi.org/10.17504/protocols.io.j8nlk9ndwv5r/v1
  • 1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology;
  • 2MPI EVA
  • MPI EVA Ancient DNA Core Unit
Julia Zorn
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DOI: dx.doi.org/10.17504/protocols.io.j8nlk9ndwv5r/v1
Document Citation: Julia Zorn, Ancient DNA Core Unit 2025. DeckLayout: automated in-solution hybridization capture (MPI-EVA method). protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk9ndwv5r/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: January 06, 2025
Last Modified: January 13, 2025
Document Integer ID: 117725
Funders Acknowledgements:
Max Planck Society
Abstract
This document provides an overview of the in-solution hybridization capture deck layout for Hamilton workstations. It is intended to help users in setting up or modifying their existing ML STAR systems. Users are advised to consult Hamilton Company to acquire the necessary carriers, modules, inserts, and accessories required for proper system configuration.
Deck layout: automated in-solution hybridization capture (MPI-EVA method)

When setting up a Hamilton workstation, please consult Hamilton Company to obtain the required carriers, modules, inserts, and other accessories. The following documentation provides an overview of the in-solution hybridization capture deck layout, designed to assist you in setting up or modifying your existing ML STAR system.


Hamilton work station - NGS STAR MOA (custom-specific configuration: ML STAR with 8 independent channels, MPH96, 5 HHS, 3 CPAC). Please pay attention to build in adapters on Hamilton Heater Shakers (HHS). Required add-ons are: iSWAP Plate Handler (for full-deck plate transport), MPH Extension Left Standard (for usage of MPH96) and the ODTC (for on-deck thermal cycling of 96 well plates).


picture of empty deck (basic setup for in-solution hybridization capture (MPI-EVA))

picture of empty deck (basic setup for in-solution hybridization capture (MPI-EVA))

Tube adapter for 24x 2ml Tubes for CPAC, supplier: Hamilton company

Tube adapter for 24x 2ml Tubes for CPAC, supplier: Hamilton company

add-on option: trough lid for 60mL reservoirs, supplier: Hamilton company (Hamilton APE solution).

add-on option: Cytomat integration with transfer station (Hamilton APE solution) to compensate for higher tip consumption without need for user refill of system.