- Multi-channel pipet (40 µL)
- Vortex + benchtop centrifuge (for tubes and PCR strips)
- DG8 cartridge holder (ref. Bio-Rad: 1863051)
- PX1 PCR plate sealer (Bio-Rad)
- QX200 droplet generator (Bio-Rad)
- QX600 or QX200 droplet reader (Bio-Rad)
- Specific DNA-workstation (sterile area equipped with air filtration and UV systems)
All tubes and tips must be sterile
- 1000 µL tips with filter
- 100 µL tips with filter
- PCR strips (one for 8 samples)
- 0.5 mL / 1.5 mL / 2 mL / 5 mL tubes according to the number of samples to be analysed
- DG8 gaskets for ddPCR (one for 8 samples) (ref. Bio-Rad: 1863009)
- DG8 cartridge for ddPCR (one for 8 samples)(ref. Bio-Rad: 1864008)
- ddPCR 96-well plate (ref. Bio-Rad: 12001925)
- Pierceable foil seal (ref. Bio-Rad: 17005225)
For any manipulation in a rare DNA room, provide complete equipment (disposable coat, cap, mask, shoe covers & gloves).
- DNase and RNase free water
- 2x ddPCRSupermix for Probes (No dUTP) (ref. Bio-Rad: 1863023 or 1863024)
- Specific forward and reverse primers (50 µM)
- Probe marked with fluorescent dye (50 µM)
- Digestion enzyme and buffer CutSmart (if applicable)
- Droplet generation oil (ref. Bio-Rad: 1863004)
- DNA samples extracted, diluted or not