Amplify the resistance cassette for knocking out a genetic region of interest using the appropriate primer pairs. Use a high-fidelity polymerase, such as Q5, KAPA, or Phusion according to manufacturer instructions.
Amplify the resistance cassette for knocking out a genetic region of interest using the appropriate primer pairs. Use a high-fidelity polymerase, such as Q5, KAPA, or Phusion according to manufacturer instructions.