Mar 21, 2024

Public workspaceDark Light Box

DOI
dx.doi.org/10.17504/protocols.io.ewov19jz7lr2/v1
  • 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Eileen Ruth Torres
Open access
DOI: dx.doi.org/10.17504/protocols.io.ewov19jz7lr2/v1
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2024. Dark Light Box. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov19jz7lr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 20, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 97021
Keywords: ASAPCRN, behavior, anxiety, mouse model
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Behavioral test for anxiety-like behaviors in mice.
Materials
  • 45 x 20 x 20cm custom box divided into 1/3 dark (covered with lid, <5lux) and 2/3 white (~100lux)
  • video recording set up
  • cleaning solution
Set up the dark light box. See materials.
Place each mouse into the dark compartment before closing the lid and left to explore for 6 minutes.
Video record each experiments.
Manually score for each animal the time in the light box as well as the number of times the mouse entered the light box.