Feb 13, 2024
Cylinder test in rats
- Eduard Bentea1,
- María Sanchiz Calvo1,
- Veerle Baekelandt1
- 1Katholieke Universiteit Leuven
Protocol Citation: Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt 2024. Cylinder test in rats. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx36zdg8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 13, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95156
Keywords: ASAPCRN
Funders Acknowledgement:
ASAP (Aligning Science Across Parkinson's)
Abstract
Protocol for performing the cylinder test in rats. The cylinder test evaluates asymmetry in forelimb use, and can be used in animal models with unilateral lesions of the nigrostriatal dopaminergic pathway.
Test
Test
1h
1h
Bring cages to the behavioral room for at least 01:00:00 prior to the test for habituation
1h
Place each rat in a glass cylinder (20-cm wide), surrounded by mirrors to allow a full 360 degree view. Videotape from the front for 00:05:00 or until the rat performs a minimum of 20 weight-bearing forepaw contacts with the cylinder walls.
5m
Perform blinded analysis from the recorded videos of the number of contralateral forepaw contacts, and express these as a percentage of total forepaw contacts.