Protocol Citation: Sabina Marciano, Roberta Marongiu 2023. Cylinder Test. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxxpnlpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 86457
Keywords: ASAPCRN
Abstract
The Cylinder test is used to evaluate locomotor asymmetry in rodent models
Materials
- Glass cylinder 20 cm in diameter 30 cm high
- Angled mirror
- 70% ethanol for cleaning between each session
Place the mouse in the transparent cylinder for 00:05:00
5m
Place an angled mirror at the back of the cylinder to allow observation of behavior from every angle
Record mouse behavior for 00:05:00
Main outcome measures: Left/Right contacts, Number of rears, L/R hind-paw contacts, Total time rearing, Time on the ground
5m