Sep 13, 2022
Version 2
Cylinder behavioral test (mice version) V.2
- 1Vall d'Hebron Research Institute
Protocol Citation: Marta Gonzalez-Sepulveda, Joan Compte, Miquel Vila 2022. Cylinder behavioral test (mice version). protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkw58dl5r/v2Version created by joan.compte
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 13, 2022
Last Modified: November 10, 2023
Protocol Integer ID: 69932
Abstract
Cylinder behavioral test for left and right forepaw use in mice
Guidelines
Important: Rats that present an asymmetric usage of the right-left forepaws at basal examination need to be excluded from the experiment.
Before start
All behavioral tests have to be performed during the light cycle by an investigator blinded to the experimental groups.
First allow mice to habituate to the experimental room for at least 1 h before each test
Put animals individually in a glass cylinder and the total number of left and right forepaw touches performed within 5 min was counted
Clean behavioral equipment with 70% ethanol after each animal has been tested to avoid olfactory cues