A | B | C | |
Item | Vendor | Catalog # | |
DMEM, high glucose | Thermo Fisher | 11965118 | |
DPBS w/o Calcium and magnesium | Corning | MT21031CV | |
Fetal Bovine Serum (FBS) | Corning | 35-011-CV | |
L-Glutamine | Sigma | G8540 | |
MEM Non-Essential Amino Acids (100X) | Thermo Fisher | 11140050 | |
0.25% Trypsin with EDTA | Thermo Fisher | 25200114 | |
Opti-MEM | Thermo Fisher | 31985062 | |
Sodium Pyruvate 100 mM | Thermo Fisher | 11360070 | |
Lipofectamine 2000 | Thermo Fisher | 11668019 |
A | B | |
DMEM, high glucose | 385 ml | |
Fetal Bovine Serum (FBS) | 50 ml | |
L-Glutamine (100X) | 5 ml | |
MEM Non-Essential Amino Acids (100X) | 5 ml | |
Sodium Pyruvate 100 mM | 5 ml |