Dec 13, 2023
Cryopreservation of intestinal/colonic biopsies
- 1Children's Hospital of Philadelphia, Gastrointestinal Epithelium Modeling Program
Protocol Citation: Tatiana Karakasheva 2023. Cryopreservation of intestinal/colonic biopsies. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyj43qlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 12, 2023
Last Modified: December 13, 2023
Protocol Integer ID: 92223
Funders Acknowledgement:
Children's Hospital of Philadelphia Research Institute
Abstract
This is a protocol for cryopreservation of biopsies, optimized for maximum viability and establishment of primary lines at a later point. Specific notes included for cryopreservation of samples for future scRNA-seq.
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