Low passage NIH-3T3-flpin cells (Life Technologies)
DMEM medium with Glutamate (Hyclone)
100X Non essential amino acids (Hyclone)
100X Penicillin-Streptomycin (Hyclone)
100X Sodium Pyruvate (Hyclone)
Fetal bovine Serum (FBS, Sigma)
Molecular Biology reagents:
BRIE gRNA library (Addgene #73633)
Endura Duo electro-competent cells (Lucigen)
Gene Pulser II for electroporation (Biorad #165-2105)
Machery-Nagel kit (Maxi-prep for plasmids)
Electroporation Cuvette 0.1mm (Biorad)
Rabbit anti-pRab10 (Clone MJF-R-22-5)
Donkey anti-rabbit Alexa-647H+L (Thermo Fisher)
Qiagen genomic DNA mini-kit
Proteinase K 20mg/ml (Zymo)
DNA mini-columns (Econospin)
10mM dNTPS (Thermo Fisher)
AMPure XP reagent (Beckman Coulter)
Nuclease free water (Thermo Fisher)
Sterilized centrifuge flasks
PCR library amplification primers