Protocol Citation: Kamaljot Gill, Aradhana Sachdev, Bruce Conklin, Claire D Clelland 2022. Creating iPSC lines with Ribonucleoprotein (RNP): Nucleofection, Single-cell Sorting, Genotyping, and Line Maintenance Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2oeopv1y/v1 License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Protocol status: WorkingWe use this protocol and it’s working.
Created: May 10, 2022
Last Modified: May 20, 2022
Protocol Integer ID: 62319
Keywords: iPSC lines , Ribonucleoprotein (RNP), gene editing , nucleofection, single-cell sorting, FACS, genotyping, spCas9, Line Maintenance, Stem Cells, excision