Protocol Citation: Bjorn Bartholdy, a.g.henry 2022. CPMU. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld96dng5b/v2Version created by Bjørn Peare Bartholdy
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 10, 2021
Last Modified: December 09, 2022
Protocol Integer ID: 55835
Funders Acknowledgement:
ERC European Union’s Horizon 2020
Grant ID: STG–677576
Abstract
Mineralising solution for the oral biofilm. From Sissons et al. 1991.
Materials
Chemicals
dH2O
CaCl2
NaH2PO4
MgCl
Urea
Equipment
1000 mL beaker
Positive flow fumehood (or other starch-free environment)
Protocol materials
Sodium FluorophosphateMerck MilliporeSigma (Sigma-Aldrich)Catalog #344443
Step 2
UreaP212121
Step 2
Sodium Phosphate monobasic
Step 2
Magnesium ChlorideFisher ScientificCatalog #AC223210010
Step 2
Calcium Chloride
Step 2
Before start
This solution MUST be prepared in a sterile and starch-free environment.
Add 300 mL distilled (or deionized) dH2O to a 1000 mL beaker, with stirring and heat 60 °C
Add:
- 1.55 g Calcium ChlorideSigma Aldrich
- 1.44 g Sodium Phosphate monobasicSigma Aldrich
- 0.72 g Sodium FluorophosphateSigma AldrichCatalog #344443
- 0.08 g Magnesium ChlorideSigma AldrichCatalog #AC223210010
- 30 g UreaSigma Aldrich
Add the remaining 700 mL and keep stirring until precipitate has completely dissolved
Store in fridge at 4 °C
Note
The solution should be filter-sterilised before storing!