Apr 20, 2022

Public workspaceCounting kidneys

DOI
dx.doi.org/10.17504/protocols.io.6qpvr6k4pvmk/v1
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DOI: dx.doi.org/10.17504/protocols.io.6qpvr6k4pvmk/v1
Protocol Citation: Gabriel J Barrero, Abraham Palmer, Oksana Polesskaya 2022. Counting kidneys. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr6k4pvmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 11, 2022
Last Modified: April 20, 2022
Protocol Integer ID: 60610
Abstract
Dissecting and counting kidneys in rats.
Rats are euthanized as per IACUC guidelines
Spray fur with ethanol. Pick up skin above the sternum, and make a cut through skin and muscle layers.
Don’t cut the chest cavity (don’t go above the diaphragm).
Cut skin and muscles from the sternum down to the lower belly. Mostly you will see liver and colon.


Lift liver lobes and push aside fat tissue and intestines. You will see dark-colored organs – kidney and spleen.
Gross dissection
Representative renal anomalies observed in rats. A. Grossly normal renal development. B. Unilateral renal agenesis (URA) PloS one. 10. e0118147. 10.1371/journal.pone.0118147

Count the number of observed kidneys and make a record in the file.