Purified recombinant antibody, 0.9–1.1 mg/mL
SimplyBlue SafeStain, Thermo Fisher, LC6060
4-12% NuPage Novex bis-tris mini gel, 1 mm thick, 10-well, Invitrogen NP0321BOX
20x MOPS SDS running buffer, Invitrogen NP0001
4x NuPage sample buffer, Invitrogen NP0007
10x NuPage sample reducing agent, Invitrogen NP0009
PageRuler Plus Prestained protein ladder, Invitrogen 26619 (optional)
Microcentrifuge tubes, Neptune 3745.X
Pipette tips, 1000 µL, VWR 76322-154
Pipette tips, 10 µL, VWR 76322-132
Pipette tips, 200 µL, VWR 76322-150
Pipette tips, 20 µL, VWR 76322-134
Pipettes, 10 mL, VWR 89130-898
Pipettes, 25 mL, VWR 89130-900
Pipettes, 5 mL, VWR 89130-896
Pipettes, 50 mL, VWR 89130-902
Gel loading tips, Corning 4853
PBS, 1X pH 7.4, VWR 45000-446
250mL sterile bottles, Corning 430281
Deionized water
IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard