This protocol describes day 5 of our workflow to grow worms in liquid culture to induce the production of natural products (in this case, 1-HP derivatives). In this protocol, we get an approximate count for the number of life-stage synchronized worms exposed to the toxin, and we collect the supernatant, which contains 1-HP and its derived metabolites. After collecting the supernatant, we freeze and lyophilize it to prepare for extraction.