Once the machine is finished priming, remove the stage insert and gasket and discard the blank coverslip. Carefully load the sample coverslip into the stage plate, place gasket on top, and tighten the stage insert. Remove any buffers or salts from bottom of the coverslip using a Kimwipe dipped in water. Next, situate the stage plate/stage insert into the holder of the Keyence BZ-X810 microscope, making sure fluidics lines can exit through the front of the microscope.