20 μL reaction containing:
- 2.08 µL of 10X Taq Buffer
- 0.42 µl of dNTPs (10 µM)
- 0.67 μL of each primer (10 µM)
- 0.17 μL of Taq DNA polymerase (5 units/μL)
- made up to final volume with nuclease-free water
- 30 cycles of 1 min at 94°C, 1 min at 66°C (cephx1_1), and 1 min at 72°C
20 μL reaction containing:
- 2.08 µL of 10X Taq Buffer
- 0.42 µl of dNTPs (10 µM)
- 0.67 μL of each primer (10 µM)
- 0.17 μL of Taq DNA polymerase (5 units/μL)
- made up to final volume with nuclease-free water
- 20 cycles of 1 min at 94°C, 1 min at 65°C (cephx1_2), and 1 min at 72°C