UL97_4_LEFT | TGCGCGCGGAAAGTCAG | UL97_4_RIGHT | CGGCATAACAGATCTTGTGGC | |
UL97_5_LEFT | CTCTGCGAGCTCTCTATCTCCT | UL97_5_RIGHT | AGCAGACAGCAGCCCGT | |
UL97_6_LEFT | TGGCGAGCAACAGCAGC | UL97_6_RIGHT | GCGCGCATGATCTCGCT | |
UL97_7_LEFT | TGCCACTTTGACATTACACCCA | UL97_7_RIGHT | TCCGACATGCAATAACGCCG | |
UL97_8_LEFT | TTTCCGACCCATGCCGCT | UL97_8_RIGHT | ATGCTCGCCCAGGAGACAG | |
UL97_9_LEFT | CATGGGTACGGAGGCGTTG | UL97_9_RIGHT | GGCCAACAGACGCTCCA |
UL54_1_LEFT | TGCAAAAACTTGTCCTTGCGC | UL54_1_RIGHT | ATTCTGTAACCACCGGCGTG | |
UL54_2_LEFT | GTAGTTGCACACGGCCGAC | UL54_2_RIGHT | CGTCAATCTAACCTGCCGCA | |
UL54_3_LEFT | CGTAAAAGACCCGATCCCCG | UL54_3_RIGHT | TCTCGCTGCTCTTTGAGGATC | |
UL54_4_LEFT | CTTCATCGAGTGAGAGGCGC | UL54_4_RIGHT | AGGCTTTGGTGGCGCGT | |
UL54_5_LEFT | GCTTGACGGGCTCCACAAAA | UL54_5_RIGHT | GGCGCGGTTCATCAAAGACA | |
UL54_6_LEFT | TCCCGCGTTCCCACTACATA | UL54_6_RIGHT | CAACAAGTGGGTTTCGCAGC | |
UL54_7_LEFT | ATACGGCGCACAGGGTCTT | UL54_7_RIGHT | GTGTTTGAGCCCGAGGTGG | |
UL54_8_LEFT | AGTAGCAGAGGTTGTGAGCCA | UL54_8_RIGHT | GGTTCTGTGGCGGCTATGTT | |
UL54_9_LEFT | AAACGCCGTCCTGACTCGA | UL54_9_RIGHT (V2) | CTTGCAATCTGCGCCGTC | |
UL54_10_LEFT | GGATCTGCTGTCCGTCAAAGA | UL54_10_RIGHT | ATATTGCGGGTTCGGTGGTT | |
UL54_11_LEFT | TGTTGAGCTTATAGTTGGGCGA | UL54_11_RIGHT | CGGCCTTTGTGACCGGTTAC | |
UL54_12_LEFT | CCTTATACAGGTACTCGAGGCG | UL54_12_RIGHT | GTGCTACGAGACGGGAGGA | |
UL54_13_LEFT | AAGTGCAGCCCCGACCAT | UL54_13_RIGHT | GGATCACCACGTTCGGCTG | |
UL54_14_LEFT | CCTCGATATCACAAGTCGACGC | UL54_14_RIGHT | GGCGAACTAGTGCCCGAAC | |
UL54_15_LEFT | CCGTACCCGTAGATGGAGGT | UL54_15_RIGHT | GGGACCTATTCGTTTTCACACCTA | |
UL54_16_LEFT | ACGATAGCGCGGCGACA | UL54_16_RIGHT | CGGCGTCAGCGTTTGCA |
Reagent | Volume / sample (µl) | |
Primer Pool | 2 | |
Q5‱ Polymerase | 0,25 | |
Q5 Buffer | 5 | |
H20 | 5 | |
dNTP | 0.5 | |
Sample DNA | 12,5 |
A | B | C | |
Cycles | Temperature | Time | |
1 | 98°C | 30’’ | |
5 | 98°C | 10’’ | |
65°C | 3’ | ||
30 | 98°C | 10’’ | |
65°C | 30’’ | ||
72°C | 2’ | ||
1 | 72°C | 2’ | |
1 | 4°C | ∞ |