| Plasmid
| Source or reference
| Cloning primers
|
| ICDm-His
| this
work
| fw: aattcgCTAGCgccaccAtgggctgcgtgcagtgcaaagataaagaagcgcaggcaccacctgca
rev: ggtggtgcctgcgcttctttatctttgcactgcacgcagcccaTggtggcGCTAGcg
|
| ICDm-S351A-His
| this work
| Mutagenesis primers:
fw: CTGAAGCGAGCCgcCGTGGATGTGGAC
rev: GTCCACATCCACGgcGGCTCGCTTCAG
Extension primers:
fw: same as for PLPPR3-ICDm-His
rev: gtgatggggccggccgtcgacgtcctggtacctcctggcc
|
| ICDc-His
| this work
| fw: aattcgCTAGCgccaccAtggCGtgcgtgcagtgcaaagataaagaagcgcaggcaccacctgca
rev: ggtggtgcctgcgcttctttatctttgcactgcacgcaCGccaTggtggcGCTAGcg
|
| N-S351A-Flag
| this work
| Mutagenesis primers:
fw: CTGAAGCGAGCCgcCGTGGATGTGGAC
rev: GTCCACATCCACGgcGGCTCGCTTCAG
Extension primers:
fw: GCTAGCgtcaccATGCTTGCTATG
rev: gtcgcggccgctTTACTTGTCATCGTCATCC
|
| N-S379A-Flag
| this work
| Mutagenesis primers:
fw: CTGCCCCGGGTCgcCACGCCCTCGCTG
rev: CAGCGAGGGCGTGgcGACCCGGGGCAG
Extension primers:
Same as above
|
| N-T380A-Flag
| this work
| Mutagenesis primers:
fw: CCCCGGGTCAGCgCGCCCTCGCTG
rev: CAGCGAGGGCGcGCTGACCCGGGG
Extension primers:
Same as above
|
| N-S379A/T380A-Flag
| this work
| Mutagenesis primers:
fw: CTGCCCCGGGTCgcCgCGCCCTCGCTG
rev: CAGCGAGGGCGcGgcGACCCGGGGCAG
Extension primers:
Same as above
|
| N-S351A/S379A/
T380A-Flag
| this work
| Same primers as for S351A and S379A/T380A mutants
|
| PLPPR3-S351A-Flag
| this work
| Same primers as for N-S351A-Flag
|
| PLPPR3-S351D-Flag
| this work
| Same primers as for N-S351D-Flag
|
| BASP1-Flag
| this work
| fw: tagagCTAGCgccaccATGGGAGGCAAGCTGAGC
rev: ccaccggatccCTCTTTGACGGCCACGCTTTGCTCGGAG
|
| pPAL_ICD-His
| Fatih Ipek
| NA
|
| pCAX_N-Flag
| Dr. Joachim Fuchs/ Dr. George
Leondaritis (Brosig et al., 2019)
| NA
|
| pCAX_Cm-Flag
|
| pCAX_Cc-Flag
|
| pCMV6_BASP1-tGFP
| #MG217147,
Origene
| NA
|
| pCAX_PLPPR3-Flag
| Brosig et
al., 2019
| NA
|
| pN1_GFP-F
| Jiang
& Hunter, 1998
| NA
|
| f(syn)-Syp-GFP-w
| Viral Core
Facility, Charité (Sampathkumar
et al., 2016)
| NA
|