A | B | |
Individual Components | ||
spermidine | 0.5 mM | |
Saponin | 0.1% (vol/vol) | |
NaCl | 50 mM | |
Tris-Cl (pH 7.5) | 10 mM | |
Store at 4ºC | ||
Add protease inhibitors just before use. |
A | B | |
Individual Components | ||
spermidine | 0.5 mM | |
NaCl | 50 mM | |
Tris-Cl (pH 7.5) | 10 mM | |
Store at 4ºC | ||
Add protease inhibitors just before use. |
A | B | |
EDTA | 20mM | |
EGTA | 40mM | |
Add 50 pg of yeast spike-in DNA to each reaction, or 50 pg/100 µl of 2X Stop Buffer. |