Healthy C57BL/6J (The Jackson Laboratory; RRID: IMSR_JAX:000664)2-3 months old mice (n=7 per experimental group)
Isoflurane (5%; Cat #: 029405, Covetrus North America)
Heparin (0.2 mL; 1000 units/mL, Cat #: 25021-400-10, Sagent Pharmaceuticals)
Paraformaldehyde (Cat #: 416780010, Acros Organics)
Bovine serum albumin (Cat #: A7906, Sigma-Aldrich)
Normal donkey serum (Cat #: 017-000-121, Jackson Immunoresearch)
Triton X-100 (Cat #: T8787, Sigma-Aldrich)
NaN3 (Cat #: S2002, Sigma Aldrich)
Anti-TH antibody, Pel-Freeze, Catalog# P40101-0, RRID: AB_461064
Anti-TH, Millipore, Catalog# AB1542, RRID: AB_90755
Anti-PGP9.5, Abcam, Catalog# ab108986, RRID: AB_10891773
Alexa Fluor 594, Invitrogen, Catalog# A-11016, RRID: AB_2534083
Alexa Fluor 488, Invitrogen, Catalog# A21206, RRID: AB_2535792
Alexa Fluor 488 secondary antibody (Donkey; Thermo Fisher, Waltham, MA, Cat# A21202, RRID:AB_141607; 1:100)
Methanol (Cat #34860: , Sigma-Aldrich)
DCM (dichloromethane) (Cat #: 270997, Sigma-Aldrich)
Ethanol (Cat #: E7023, Sigma-Aldrich)
Depex mounting medium (Electron Microscopy Sciences #13514)
Zeiss Axio Imager M2 (Item no.: 430004-0000-711)
Leica Confocal Microscope (Type: TCS SP5)