The Chromium Nuclei Isolation Kit is an all-in-one solution for the
standardized isolation of nuclei from frozen tissue for use in
10x Genomics Single Cell assays. Frozen tissue samples are
homogenized with a pestle in Lysis Buffer and passed through a
column. Next, debris is removed via centrifugation in Debris Removal
Buffer. The isolated nuclei are then washed and resuspended and
loaded directly into compatible 10x Genomics Single Cell assays.
The Chromium Nuclei Isolation Kit streamlines the nuclei isolation
process into a single workflow, allowing for increased efficiency,
scalability through sample batching, and reduced experimental
variability using 10x Genomics pre-formulated reagents. The protocol is
designed to be compatible with a wide variety of tissue types and sizes.
This User Guide outlines the process for isolating Nuclei from frozen
tissues for use in compatible 10x Genomics Single Cell assays. Refer to
the Product Compatibility and Protocol Selector pages for additional
information on choosing the appropriate nuclei isolation kit and
protocol based on the intended downstream Single Cell assay.