| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
| Cells lines | | |
| NGN2 inducible embryonic stem cells | See protocol dx.doi.org/10.17504/protocols.io.br9em93e | CVCL_9773 (modified from this source line) |
| HEK293T cells | ATCC CRL-1573 | CVCL_0045 |
| Chemicals | | |
| Dulbecco’s MEM (DMEM), F12 | | |
| Phosphate Buffered Saline 1X | Corning | 21-031-CV |
| E8 components | See protocol dx.doi.org/10.17504/protocols.io.bsacnaaw | |
| ND1 and ND2 components | See protocol dx.doi.org/10.17504/protocols.io.br9em93e | |
| Hygromycin B | Life Technologies | 10687-010 |
| | | |
| pAC150- FAM134C-GFP | This paper | Addgene 201932 |
| pAC150- TEX264-GFP | This paper | Addgene 201931 |
| pAC150- TEX264(deltaLIR, F273A)-GFP | This paper | Addgene 201930 |
| pCMV-hyPBase
hyperactive piggyBac vector | Yusa
et al 2011 | Available upon request at the Sanger
Institute Achives |
| pHAGE-TEX264-GFP | An et al 2019 | Addgene 201925 |
| pHAGE-TEX264(deltaLIR,F273A)-GFP | An et al 2019 | Addgene 201926 |
| pHAGE-mCherry-LC3B | An et al 2019 | Addgene 201924 |
| Software | | |
| Nikon Imaging Software Elements | 5.21.3 (Build 1489) | https://cellprofiler.org/ SCR_014329 |
| Cell Profiler | CellProfiler v4.0.6 | https://cellprofiler.org/ SCR_007358 |
| Fiji | ImageJ V.2.0.0 | https://imagej.net/software/fiji/ SCR_002285 |
| Hardware | | |
| Thermo Neon™ Transfection System | Thermo Fisher Scientific | MPK5000 |
| Yokogawa
CSU-X1 spinning disk confocal on a Nikon Ti-E inverted microscope | | |