| REAGENT or RESOURCE
| SOURCE
| IDENTIFIER
|
| Antibodies
|
|
|
| Human
HLA-DR(L243)-141Pr
| Biolegend
| Cat#307651, RRID:AB_2562826
|
| Human
CD19(HIB19)-142Nd
| Standard Biotools
| Cat#3142001B, RRID:AB_2651155
|
| Human
CD69(FN50)-144Nd
| Standard Biotools
| Cat#3144018B, RRID:AB_2687849
|
| Human CD4(RPA-T4)-145Nd
| Standard Biotools
| Cat#3145001B, RRID:AB_2661789
|
| Human CD64(10.1)-146Nd
| Standard Biotools
| Cat#314600B, RRID:AB_2661790
|
| Human CD226(REA1040)-147Sm
| Miltenyi
| Cat#130-126-485,
RRID:AB_2889512
|
| Human CD16(3G8)-148Nd
| Standard Biotools
| Cat#314800B, RRID:AB_2661791
|
| Human CD56(NCAM16.2)
| Standard Biotools
| Cat#3149021B,
RRID:AB_2661813
|
| ICOS (C398.4A)-151Eu
| Standard Biotools
| Cat#3151020B
|
| Human CD66b(80H3)-152Sm
| Standard Biotools
| Cat#3152011B, RRID:AB_2661795
|
| Human CD3(UCHT1)-154Sm
| Standard Biotools
| Cat#3154003B, RRID:AB_2687853
|
| Human CD11c(Bu5)-155Gd
| Standard Biotools
| Cat#314700B
|
| Human CCR4(L291H4)
| Standard Biotools
| Cat#3158032A, RRID:AB_2893003
|
| Human TIGIT(MBSA43)-159Tb
| Standard Biotools
| Cat#3159038B
|
| Human CD14(RMO52)-160Gd
| Standard Biotools
| Cat#3160006B, RRID:AB_2661801
|
| Human CD8(RPA-T8)-162Dy
| Standard Biotools
| Cat#3162015B, RRID:AB_2661802
|
| Human CRTH2 (BM16)-163Dy
| Standard Biotools
| Cat#3163003B, RRID:AB_2810253
|
| Human CD95(DX2)-164Dy
| Standard Biotools
| Cat#3164008B, RRID:AB_2858235
|
| Human LAG3(11CRC65)-165Ho
| Standard Biotools
| Cat#3165037B, RRID:AB_2810971
|
| Human CD141(M80)-166Er
| Standard Biotools
| Cat#3166017B, RRID:AB_2892693
|
| Human CCR7(G043H7)-167Er
| Standard Biotools
| Cat#3167009, RRID:AB_2661804
|
| Human CD206(15-2)-168Er
| Standard Biotools
| Cat#3168008B
|
| Human CD33(WM53)-Tm169
| Standard Biotools
| Cat#3169010B, RRID:AB_2802111
|
| Anti-biotin-170Er
| Standard Biotools
| Cat#3170003B, RRID:AB_2811234
|
| Human CD161(HP-3G10)-171Yb
| Biolegend
| Cat#339919, RRID:AB_2562836
|
| Human CXCR4(12G5)-173Yb
| Standard Biotools
| Cat#3173001B
|
| Human CD127(A019D5)-176Yb
| DVS Sciences
| Cat#3176004B
|
| Human CD47(CC2C6)-209Bi
| Standard Biotools
| Cat#3209004B
|
| Human cPARP(F21-852)-143Nd
| Standard Biotools
| Cat#3143011A, RRID:AB_2927562
|
| Human MIP1beta(D21-1351)-150Nd
| Standard Biotools
| Cat#3150004B
|
| Human IL6(MQ2-13AS)-156Gd
| Standard Biotools
| Cat#3156011B, RRID:AB_2810973
|
| Human CD68(Y1782A)-153Eu
| Biolegend
| Cat#333802, RRID:AB_1089058
|
| Human CTLA4(14D3)-161Dy
| Standard Biotools
| Cat#3161004B, RRID:AB_2687649
|
| Human OPN(polyclonal)-172Yb
| LSBio
| Cat#LS-C99283, RRID:AB_2194984
|
| Human IL1beta(CRM56)-174Yb
| Thermo Fisher
| Cat#14-7018-85, RRID:AB_468401
|
| Human TNF(Mab11)
| Biolegend
| Cat#502941, RRID:AB_2562842
|
| Biological samples
|
|
|
| Chemicals,
peptides, and recombinant proteins
| | |
| Cell-ID
20-Plex Barcoding Kit (https://fluidigm.my.salesforce.com/sfc/p/#700000009DAw/a/4u0000019iFV/_MMkFk_8Oes9hcoYhMMr6Drg2mgwF69GVV0UAFUgeQc))
| Standard Biotools
| Cat# PN 201060
|
| Maxpar
X8 Multimetal Labeling Kit-40Rxn
(https://fluidigm.my.site.com/Storefront/Cytometry/ConsumablesandReagentsCytometry/MaxparAntibodyLabelingKits/Maxpar%C2%AE%20X8%20Multimetal%20Labeling%20Kit%E2%80%9440%20Rxn?cclcl=en_US)
| Standard Biotools
| Cat#201300
|
| Smart
tube Prot1 (https://www.smarttubeinc.com/)
| Smart tube Inc.
| Cat#501351689
|
| FC
receptor blocking reagent
| Milteny Biotec
| Cat# 5170126102
|
| Bovin
Serum albumin(BSA)
| Roth
| Cat#T8844.2
|
| RPMI medium: RPMI-1640, 10% FBS
| Gibco
| Cat#31870-025
|
| PBS
| Gibco
| Cat#14200-067
|
| Methanol-free formaldehyde (FA)
solution
| Thermo Fisher
| Cat#28906
|
| Universal Nuclease
| Thermo Fisher
| Cat##88701
|
| Software and algorithms
| | |
| R
v4.3.0(Already tomorrow)
| CRAN
| https://www.r-project.org/
RRID:SCR_001905
|
| R
studio v2023.03.1-446
| Posit
| https://posit.co/download/rstudio-desktop/
RRID:SCR_000432
|
| Bioconductor
| Bioconductor- open-source
software for bioinformatics
| https://www.bioconductor.org/
RRID:SCR_006442
|
| CATALYST
| CATALYST: Cytometry dATa
anALYSis Tools.
| https://github.com/HelenaLC/CATALYST.
RRID:SCR_017127
|
| BiocManager
| Bioconductor- open-source
software for bioinformatics
| https://www.bioconductor.org/install/
|
| flowCore
| flowCore:
Basic structures for flow cytometry data
| https://www.bioconductor.org/packages/release/bioc/html/flowCore.html
RRID:SCR_002205
|
| flowWorkspace
| Infrastructure
for representing and interacting with gated and ungated cytometry data sets.
| https://www.bioconductor.org/packages/release/bioc/html/flowWorkspace.html
RRID:SCR_001155
|
| CytoML
| A
GatingML Interface for Cross Platform Cytometry Data Sharing
| https://www.bioconductor.org/packages/release/bioc/html/CytoML.html
|
| flowFP
| Fingerprinting
for Flow Cytometry
| https://www.bioconductor.org/packages/release/bioc/html/flowFP.html
RRID:SCR_001537
|
| diffcyt
| Differential
discovery in high-dimensional cytometry via high-resolution clustering
| https://github.com/lmweber/diffcyt
RRID:SCR_023006
|
| scater
| Single-Cell
Analysis Toolkit for Gene Expression Data in R
| https://www.bioconductor.org/packages/release/bioc/html/scater.html
RRID:SCR_015954
|
| Ggplot2
| The R Graph Gallery
| https://r-graph-gallery.com/ggplot2-package.html
RRID:SCR_014601
|
| CyTOF
Software v7.1
| Fluidigm
| https://go.fluidigm.com/cytofsw/v7
RRID:SCR_021055
|
| FlowJo
v10.9
| FLOWJO
| https://www.flowjo.com/solutions/flowjo/downloads
RRID:SCR_008520
|
| Other
| | |
| 5ml
serological pipettes
| Eppendorf
| Cat#0030127714
|
| 1.5Ml
Eppendorf tube
| Eppendorf
| Cat#50-809-150
|
| Falcon
Tubes
| A Corning Brand
| Cat#352096
|
| Water
bath at 37°C
| Memmert
| Cat#WNB 45
|
| Centrifuge
| Eppendorf
| Cat #5418R
|
| filter
tips
| Starstedt
| Cat#70.3050255
|