Apr 06, 2023
Cell viability assessment
- Qing Wang1,2
- 1Massachusetts General Hospital;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network
Protocol Citation: Qing Wang 2023. Cell viability assessment. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly7rpelx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 06, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 80147
Keywords: ASAPCRN
Funders Acknowledgement:
From cancer associations to altered immunity in the pathogenesis of Parkinson's disease
Grant ID: ASAP-000312
Abstract
This is the protocol for assessing cell viability using a MTS cell proliferation assay kit.
1. Cells are seeded and differentiated for 4 days; assess cell viability using an MTS cell proliferation assay kit (ab197010, Abcam).
2. Treat the differentiated cells with synthetic DOPA pheomelanin or synthetic DOPA eumelanin or vehicle PBS to determine the time and dose responses of the cells to the treatment.
3. Remove culture medium and add a 110 µL solution containing 10 µL MTS and 100 µL medium to each well, incubate in humidified air containing 5% CO2 at 37°C for 1 hour.
4. Measure absorbance at 490 nm using a microplate reader.
Protocol references
Su, W., Zhu, S., Chen, K., Yang, H., Tian, M., Fu, Q., ... & Yang, C. (2021). Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer.Journal of Experimental & Clinical Cancer Research,40(1), 1-15.
Ye, Q., Srivastava, P., Al-Kuwari, N., & Chen, X. (2022). Oncogenic BRAFV600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase.Neural Regeneration Research.